|
KMID : 0861020160310040001
|
|
Korea Journal of Herbology
2016 Volume.31 No. 4 p.1 ~ p.10
|
|
|
Research of Efficacy of Curculiginis Rhizoma aquaous extract on collagen induced arthritis
|
|
Seo Bu-Il
Roh Seong-Soo
Park Ji-Ha
Park Chan-Ik
Koo Jin-Suk
|
|
|
Abstract
|
|
|
Objectives：In Korean medicine, Curculiginis Rhizoma was treated for arthritis in remedy. But efficacy of Curculiginis Rhizoma on collagen induced arthritis was not revealed.
Methods：Anti inflammatory effect of Curculiginis Rhizoma was researched in vitro with RAW264.7 cell and cell toxicity, levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-12) and PGE2 were analyzed by ELISA assay. Inflammatory protein were analyzed by western blotting assay (JNK, ERK, COX-2, TNF-α and IL-1β). In vivo, collagen induced arthritis mice model was used to evaluate anti-inflammation effect through arthritis index, immune cell number and cytokine levels (TNF-α, IL-6 and IL-1β) in serum.
Results：ECR(Extract of Curculiginis Rhizoma) has not shown cell toxicity in 200 ㎍/㎖ on RAW264.7 cell. ECR suppressed releases of NO, TNF-α, IL-1β, IL-6, IL-12 and PGE2 on RAW264.7 cell treated with lipopolysacharide (1 ㎍/㎖). And ECR inhibited regulation of TNF-α, IL-1β and IL-6 mRNA, reduced protein release of JNK, ERK, iNOS, COX-2, IL-1β and TNF-α. AI of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly decreased compared to vihicle arthritis mice, the number of immune cell in foot joint was increased on control mice but those of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly reduced. This results correspond with contens of cytokines (TNF-α, IL-1β and IL-6) in serum.
Conclusions：Curculiginis Rhizoma has anti-inflammation effect on RAW264.7 cell in vitro and collagen induced arthritis in vivo . So it is necessary to research more mechanism for cascade imfact.
|
|
|
KEYWORD
|
|
|
Curculiginis Rhizoma, anti-inflammation, collagen, arthritis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|